Next meeting : Thursday November 29th 2012, 10:00am – iRTSV

Fall is back and our next meeting is approaching ! Please note that the date changed, and the meeting will be on Thursday November 29th 2012, at 10:00 am at the iRSTV.

The location is the Maison Minatec (see below for more details, access map here).

Epigenetics NovemberThe speakers are :
Seminar by A. Arneodo (ENS Lyon)
Master replication origins at the heart of the organization and fragility of the human genome

Short talk by Edwige Hiriart (Verdel Lab, IAB)
RNA directed gene silencing in fission yeast

Short talk by Matthieu Benoit (Plevin Lab, IBS)
Insights into the recognition of an oncogenic microRNA precursor by the key cofactor TRBP during human microRNA biogenesis

Coffee break and lunch will be provided by Active Motif.

Please help us advertise the event! The full resolution flyer is accessible here.

Location : Maison Minatec, CEA Grenoble
This conference room is on the CEA campus, and the entrance is free !!

Located in front of the tram stop “Cité Internationale”, it is particularly easy to reach using public transportations (tramway, bus or train). It also has two parking lots nearby.

Click here to have a full size map.

Detailed program
10:00 Seminar by Dr. A. Arneodo (ENS Lyon)
Master replication origins at the heart of the organization and fragility of the human genome

In higher eukaryotes, the absence of specific sequence motifs marking the origins of replication has been a serious hindrance to the understanding of the mechanisms that regulate the initiation and the maintenance of the replication program in different cell types. During the course of evolution, mutations do not affect equally both strands of genomic DNA. In mammals, transcription-coupled nucleotide compositionalskews have been detected but no compositional asymmetry has been associated with replication.
In a first part, using a wavelet-based multi-scale analysis of human genome skew profiles, we identify a set of one thousand putative replication initiation zones. We report on recent DNA replication timing data that provide experimental verification of our in silico replication origin predictions.
In a second part, we examine the organisation of the human genes around the replication origins. We show that replication origins, gene orientation and gene expression are not randomly distributed but on the opposite are at the heart of a strong organisation of human chromosomes. The analysis of open chromatin markers brings evidence of the existence of accessible open chromatin around the majority of the putative replication origins that replicate early in the S phase.
We conclude by discussing the possibility that these “master’ replication origins also play a key role in genome dynamics during evolution and in pathological situations like cancer.

11:00 Short talk by Edwige Hiriart (Verdel Lab, IAB)
RNA directed gene silencing in fission yeast

11:30 Short talk by Matthieu Benoit (Plevin Lab, IBS)
Insights into the recognition of an oncogenic microRNA precursor by the key cofactor TRBP during human microRNA biogenesis

Micro-RNAs (miRNA) are small non-coding RNAs that regulate gene expression through RNA interference (RNAi). Human miRNAs are generated via a series of enzymatic processing steps. The precursor miRNA (pre-miRNA) is recognized and cleaved by a complex containing the RNase III enzyme, Dicer, and several non-catalytic accessory proteins. HIV TAR element binding protein (TRBP) is a constituent of the Dicer complex which augments complex stability and potentially functions in substrate recognition and product transfer to the RNA-induced silencing complex (RISC).
Here we have analyzed the interaction between the RNA-binding region of TRBP and the oncogenic human miRNA, miR-155, at different points in the miRNA biogenesis pathway. We show that the region of TRBP that binds miRNA precursors comprises two independent double-stranded RNA binding domains (dsRBDs) connected by a 60 residues flexible linker. No evidence of contact between the two dsRBDs was observed either in the apo form or in the RNA-bound state. The RNA-binding region of TRBP interacts non-cooperatively with pre-miR-155 and the related Dicer product miR-155/miR-155* and can form a complex with two protein molecules per RNA. Finally, we determined that the RNA-binding region of TRBP interacts with pre-miR-155 and miR-155/miR-155* via the same binding surface and with similar affinity, suggesting that the RNA binding region of TRBP could function before and after processing of pre-miRNAs by Dicer.

12:00 Lunch provided by Active Motif.

Second meeting: we were more than 100 participants!

The second Meeting has been a great success! Talks were exciting, lots of questions, and the conference room was full!

We were more than 100 participants! Thank you so much for your participation!

We also wish to thank Active Motif for sponsoring our meetings with great drinks and food.

Naturally, any comment / suggestion is very welcome! Use the contact page or email us directly !

And save the date for the next meeting: Nov 15th, at the iRTSV. We will soon email you with more information about it !

Cheers,
Jérôme, Jan and André

Second meeting : Thursday June 21th 2012 – IAB

Our second meeting will be on Thursday, June 21th 2012 10am at the IAB (access).

201206_Meeting2The speakers will be :
Seminar by Dr. Ali Hakimi (Institut J. Roget)
Toxoplasmagondii and subversion of its human host cell epigenome : the price to pay to survive

Short talk: Dr. Jan Kadlec (EMBL)
Structural studies on the Dosage Compensation Complex

Short talk: Dr. Anouk Emadali (iRTSV / IAB)
BET bromodomain inhibition increases therapeutic response in lymphoma

Coffee break and lunch will be provided by Active Motif, who is sponsoring our meetings.

Please help us advertise the event! The flyer can be found here.

1st meeting: You made it a Success!!!

Thank you so much for your participation in the first Meeting!

It was really exciting to have our community gathered around great speakers and sharp questions. And of course good chocolate candies!

More to come, we will soon email you more details about the next meeting !!

Cheers,

Jérôme, Jan and André

First Meeting : March 15th 2012

We are pleased to confirm that our first meeting will be held on Thursday, March 15th 2pm at the IBS (access).

201203_Meeting1The speakers will be :
Seminar by Dr. Saadi Khochbin (IAB)
Molecular basis of post-meiotic male genome reprograming

Short talk: Dr. Jordi Xiol (EMBL)
Involvement of the chaperone machinery in the mouse and insect piRNA pathway

Short talk: Dr. Cristel Carles (iRTSV)
Developmental programs and cell fates in plants: from genetics to epigenetics

 

Refreshments and finger food will be provided by Active Motif, who kindly accepted to sponsor our meetings.

Please help us advertise the event! The flyer can be found here.

Launch !

Dear colleagues,

The Grenoble scientific community has been demonstrating a growing interest in the field of chromatin dynamics, transcription regulation and epigenetics in general. We propose to create a local “Chromatin, Transcription and Epigenetics Club”.

Several times a year, local actors would gather together, to facilitate our interactions, and hopefully spark off new collaborative projects. Topics covered would range from chromatin dynamics to transcription regulation, and epigenetics using a broad variety of models and methodologies, from biochemistry and cellular biology to structural biology, and Next-Gen sequencing.

We propose to organize three meetings a year (autumn, winter and spring). Meetings would consist of three presentations. Two PhD students/ Post Docs would present their work in 20 min, followed by 10 min of questions. After a break, a senior scientist would present a seminar of 45 min, followed by 15 min of questions. The location of the meetings would rotate between iRTSV, IBS / EMBL and IAB.

An initial organizing committee is composed of Jerome Govin (chromatin dynamics in gametes, iRTSV), Jan Kadlec (X chromosome dosage compensation, EMBL) and André Verdel (RNAi and cell differentiation, IAB).

Initial discussions with some of you are very encouraging. A first tentative list of potentially interested labs has been made. Please read, correct and amend this list considering your respective network. We welcome any ideas!

As soon as initial feedback has been gathered, we will start to organize our first meeting, hopefully for February 2012.

Sincerely yours,

Jérôme, Jan and André.